کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3074541 1188880 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A responsive MRI contrast agent to monitor functional cell status
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب شناختی
پیش نمایش صفحه اول مقاله
A responsive MRI contrast agent to monitor functional cell status
چکیده انگلیسی

It has been shown that insoluble Gd chelates are suitable MRI contrast agents for conditional activation by intracellular lipases. The DTPA-based, insoluble, inactive contrast agent was internalized into dendritic cells by phagocytosis. Cleavage of long aliphatic side chains by intracellular lipase activity leads to the contrast agents solubility and hereby its activation depending on the enzyme expression. Uptake and activation of the contrast agent was much reduced in Flt3+ CD11b+ progenitor cells. Detectability limits in the T1-weighted MR images were estimated in phantoms and in vivo in the rat brain. Marginal toxic effects were only observed at very high concentrations of the contrast agent. The chelate can easily be modified to be targeted by enzymes expressed during specific change of cell status like activation or differentiation. Such a system is suitable for functional cellular in vivo MR imaging.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: NeuroImage - Volume 32, Issue 3, September 2006, Pages 1142–1149
نویسندگان
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