Simultaneous Determination of Four Substances in Plasma and Dermal Microdialysates of Guinea Pig after Different Acupoints Administration of Fufang Baijiezi Gel

ObjectiveTo study the pharmacokinetic properties of Fufang Baijiezi Gel (FBG) after different acupoints administration.MethodsSinapine thiocyanate, tetrahydro-palmatine, 6-gingerol, and asarinin, which were four substances of FBG, were determined by a sensitive liquid chromatography tandem mass spectrometry method (LC-MS) both in plasma and dermal microdialysates of guinea pig simultaneously. Microdialysates were separated on an Ultimate® XB-Phenyl analytical column (150 mm × 2.1 mm, 5µm) and detected by electrospray ionization (ESI) in selected ion monitoring (SIM) mode. The method was validated in terms of selectivity, linearity, sensitivity, and recovery. Result A significant difference was observed in main pharmacokinetic parameters of Cmax, tmax, and AUC between acupoints administration and nonacupoints administration.ConclusionAcupoints administration resulted in a more obvious increase in bioavailability of sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin than nonacupoints administration.