Simultaneous Determination of Four Active Ingredients in Wuji Pellet by HPLC

ObjectiveTo apply the high performance liquid chromatography (HPLC) method to the determination of four ingredients including paeoniflorin, berberine, evodiamine, and rutaecarpin in Wuji Pellet.MethodsUsing Thermo-ODS2-Hypersil column (250 mm × 4.6 mm, 5 μm) and taking acetonitrile-0.05 mol/L monopotassium phosphate as mobile phase for elution, the conditions were as follows, flow rate of 1.0 mL/min, UV detector wavelength of 225 nm, and column temperature of 25 °C.ResultsThe standard curves of paeoniflorin, berberine, evodiamine, and rutaecarpine showed a good linear relationship in 0.0124—0.124, 0.0349—0.349 mg/mL, and 0.6—6, 0.5—5 μg/mL. The average recovery rates were 99.45%, 97.69%, 98.18%, and 98.46%, respectively.ConclusionThe method is of high sensitivity and nice reproducibility, and it could be used for the simultaneous determination of the four ingredients in Wuji Pellet.