Fingerprint Analysis of Codonopsis Radix by HPLC Coupled with Chemometrics Analysis

ObjectiveTo establish a validated high performance liquid chromatography (HPLC) for the fingerprint analysis of Codonopsis Radix and for the determination of lobetyolin.MethodsHPLC coupled with diode array detection method was employed to establish the fingerprint profile and quantitative determination of lobetyolin in Codonopsis Radix. Principal component analysis method was employed to analyze the 52 Codonopsis Radix samples.ResultsThe reference chromatogram was generated with 25 common peaks showing good separation from adjacent peaks.ConclusionStatistical analysis of the obtained data demonstrates that the developed HPLC fingerprint combined with chemometric is a reliable method for the similar evaluation and quality assessment of Codonopsis Radix, and other traditional Chinese herbs.