Prophylaxis and Therapy of Isaria felina on Acute Renal Failure Induced by Glycerin in Rats

ObjectiveTo evaluate the prophylaxis and therapy of Isaria felina (IF) on glycerin-induced acute renal failure (ARF) in rats.MethodsForty male Wistar rats were divided into control, model, Uremic Clearance Granule (UCG, positive control), high- and low-dose IF groups. Rats in the high- and low-dose IF groups were ig administered with 200 and 100 mg/kg IF, respectively, while rats were ig administered with 3.6 g/kg UCG successively for 7 d to establish UCG group. The rats in model, control, and drug-treated groups were im injected with 8 mL/kg 50% glycerin after drinking was quitted for 24 h to induce ARF in rats. The drugs were continued to give thereafter. The level of blood urea nitrogen (BUN) and serum creatinine (SCr) were determined 24 and 72 h after the injection of glycerin, also the kidney was dissected for pathology examination.ResultsIm injection with 8 mL/kg 50% glycerin could successfully induce ARF in rats. The dose of 200 mg/kg IF could reduce the high levels of BUN and SCr, and ameliorate the pathological damage of the kidney.ConclusionIF has good protective and therapeutic effects on ARF and it is a potential and valuable Chinese herb for ARF.