Simultaneous Determination of Seven Flavonoids in Aerial Parts of Artemisia frigida by HPLC

ObjectiveTo establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida.MethodsHypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min.ResultsAll calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6).ConclusionThe method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.