Saponins from Roots of Panax notoginseng

ObjectiveTo study the chemical constituents in the dried roots of Panax notoginseng.MethodsThe constituents were isolated and purified with chromatographic methods. Their structures were elucidated by spectroscopic methods (1D, 2D NMR, UV, IR, [α]D, and HRESI-TOF-MS) and chemical analyses.ResultsTwenty saponins including 20(S)-ginsenoside Rh1 (1), 6-O-β-D-(6′-acetyl)-glucopyranosyl-24-ene-dammar-3β, 6α,12β,20S-tetraol (2), ginseno- side Rf (3), notoginsenoside R2 (4), ginsenoside Rg2 (5), ginsenoside Rg1 (6), notoginsenoside Rt (7), koryoginsenoside R1 (8), 6-O-(β-D-glucopyranosyl)-20-O-(β-D-xylopyranosyl)-3β,6α,12β,20(S)-tetrahydroxy-dammar-24-ene (9), pseudoginsenoside Rt3 (10), notoginsenoside R1 (11), ginsenoside Re (12), notoginsenoside N (13), ginsenoside F1 (14), ginsenoside U (15), ginsenoside Rk3 (16), 3β,12β-dihydroxydammar-(E)-20(22),24-diene-6-O-β-D-xylopyranosyl-(1 → 2)-β-D-glucopyranoside (17), ginsenoside Rh4 (18), pseudoginsenoside Rt5 (19), and vinaginsenoside R22 (20) were obtained.ConclusionCompounds 2, 19, and 20 are isolated from this species for the first time. The 1H-NMR data of compound 19 and 1H-NMR and l3C-NMR data of compound 20 are first reported. Meanwhile, the NMR data of β-D-xylopyranosyl group in compound 9 is corrected.