کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3107107 1581747 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improved neurite outgrowth on central nervous system myelin substrate by siRNA-mediated knockdown of Nogo receptor
ترجمه فارسی عنوان
رشد نوریت بهبودیافته در زیرلایه میلین سیستم عصبی مرکزی توسط مهار بواسطه siRNA توسط گیرنده Nogo
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی مراقبت های ویژه و مراقبتهای ویژه پزشکی
چکیده انگلیسی

PurposeTo investigate the in vitro effect of short interfering RNAs (siRNAs) against Nogo receptor (NgR) on neurite outgrowth under an inhibitory substrate of central nervous system (CNS) myelin.MethodsThree siRNA sequences against NgR were designed and transfected into cerebellar granule cells (CGCs) to screen for the most efficient sequence of NgR siRNA by using reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence staining. NgR siRNA sequence 1 was found the most efficient which was then transfected into the CGCs grown on CNS myelin substrate to observe its disinhibition for neurite outgrowth.ResultsCompared with the scrambled control sequence of siRNA, the NgR siRNA sequence 1 significantly decreased NgR mRNA level at 24 h and 48 h (p < 0.05), which was recovered by 96 h after transfection. NgR immunoreactivity was also markedly reduced at 24 and 48 h after the transfection of siRNA sequence 1 compared with that before transfection (p < 0.05). The NgR immunoreactivity was recovered after 72 h post-transfection. Moreover, the neurite outgrowth on the myelin substrate was greatly improved within 72 h after the transfection with siRNA sequence 1 compared with the scrambled sequence-transfected group or non-transfected group (p < 0.05).Conclusion: siRNA-mediated knockdown of NgR expression contributes to neurite outgrowth in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Traumatology - Volume 19, Issue 1, February 2016, Pages 16–24
نویسندگان
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