کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3134747 1584226 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nucleofection is highly efficient for transfecting genes into murine embryonic palatal mesenchymal cells in primary culture
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Nucleofection is highly efficient for transfecting genes into murine embryonic palatal mesenchymal cells in primary culture
چکیده انگلیسی

Non-syndromic cleft of the lip and/or palate is one of the most common birth defects in humans. Embryonic palatal mesenchymal (EPM) cells are an attractive source for investigating embryonic palatal development. In this study, we developed a highly efficient transfection method for murine EPM (MEPM) cells. MEPM cells were transfected with the plasmid pEGFP-N1 using two non-viral methods: nucleofection and lipofection. Nucleofection provided a much better rate of gene transfer than lipofection particularly in MEPM cells. The methylenetetrahydrofolate reductase (MTHFR) gene is an important candidate for involvement in the pathogenesis of this birth defect. The RNA interference plasmid of MTHFR was constructed and nucleofected into MEPM cells. Successful transfection resulted in a remarkable reduction in the expression of MTHFR. Taken together, the results indicate that nucleofection is highly efficient for MEPM cell transfection, and that this approach may be useful for investigating gene function in the process of palatogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Oral and Maxillofacial Surgery - Volume 36, Issue 5, May 2007, Pages 429–434
نویسندگان
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