کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3146943 1197331 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytotoxicity Evaluation of a New Resin-based Hybrid Root Canal Sealer: An In Vitro Study
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Cytotoxicity Evaluation of a New Resin-based Hybrid Root Canal Sealer: An In Vitro Study
چکیده انگلیسی

ObjectiveThis study evaluated the cytotoxicity of the new experimental self-adhesive, methachrylate-based hybrid root canal sealer XT and compared it with the epoxy resin-based AH Plus Jet.MethodsThe cytotoxicity of the tested materials was evaluated after 1, 24, 48, and 72 hours by using growing and confluent mouse fibroblast cell line L929. L929 fibroblasts were maintained in Dulbecco modified medium containing 10% fetal calf serum at 37°C and 5% CO2. At confluence, cells were seeded in 24-well plates at concentration of 1.5 × 105 cells (growing cells) or 2.5 × 105 (confluent cells) for each well. An amount of 5 μL of each root sealer (mixed according to manufacturer's specifications) was placed into individual wells containing a monolayer of L929 cells to mimic the in vivo condition of the possible extrusion of sealer in the periapical tissues. Neutral Red and [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] were used for the cytotoxicity evaluation. Untreated cells were used as control. Two-way analysis of variance with Bonferroni test was used to compare the toxicity of the 2 sealers; one-way analysis of variance with Tukey test was performed to compare the cytotoxicity of each sealer at any considered time points (P < .05). Results were confirmed by examination with optical microscope.ResultsBoth sealers induced a time-dependent cell death of mouse fibroblast L929; however, XT was less cytotoxic than AH Plus Jet as indicated by viability and morphologic analyses, and its initial cytotoxicity decreased progressively over time.ConclusionsThese data support the possible use of XT as an endodontic sealer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Endodontics - Volume 40, Issue 1, January 2014, Pages 124–128
نویسندگان
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