Evaluation of Human Osteosarcoma Cell Line Genotoxicity Effects of Mineral Trixoide Aggregate and Calcium Silicate Cements

IntroductionMineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63).MethodsA mitochondrial colorimetric assay was used to evaluate the MG63 survival rate. A DNA precipitation assay was used to detect the MG63 DNA damage after contact with MTA or CS cement extracts.ResultsThe results showed that MTA and CS are cytocompatible with MG63 cells. There was no significant difference in the survival rate with MTA and CS materials (P > .05). Neither MTA nor CS cements causing MG63 cell DNA damage showed significant genotoxicity (P > .05).ConclusionsBoth MTA and CS cements are compatible with MG63 cells, and they are not cancer-causing agents.