کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3148034 | 1197385 | 2013 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing](/preview/png/3148034.png)
IntroductionThe aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections.MethodsPyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted.ResultsAnalysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P > .05).ConclusionsThe present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.
Journal: Journal of Endodontics - Volume 39, Issue 9, September 2013, Pages 1136–1140