Short-Term Cytotoxicity Assessment of Components of the Epiphany Resin-Percha Obturating System by Indirect and Direct Contact Millipore Filter Assays

The Epiphany Resin-Percha Obturating System was assessed for cytotoxicity, compared with gutta-percha and AH-Plus sealer. Specimen disks (Resilon, gutta-percha), filled glass rings (sealers), or imbibed cellulose disks (primer, thinning resin) were placed over Millipore filters in direct or indirect contact with HeLa cell monolayer, incubated for 2 hours, and stained with tetrazolium blue. Cytotoxicity was rated by the surrounding unstained zone: none (0 mm), mild (≤7 mm), moderate (7–12 mm), or marked (>12 mm). Data were analyzed with one-way ANOVA and post hoc pairwise t tests. Unstained zones indicating moderate cytotoxicity were significantly larger (p < 0.05) for Epiphany primer than for thinning resin and for freshly mixed AH-Plus than for Epiphany sealer. Set sealers (24 and 48 hours), gutta-percha, and Resilon elicited noncytotoxic responses. In conclusion, cytotoxicity of set Epiphany sealer and Resilon was comparable with that of set AH-Plus and gutta-percha. Cytotoxicity of freshly mixed Epiphany sealer, primer, and thinning resin did not exceed that of freshly mixed AH-Plus.