کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3150358 | 1197517 | 2013 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The Biological Performance of Calcium Hydroxide–loaded Microcapsules The Biological Performance of Calcium Hydroxide–loaded Microcapsules](/preview/png/3150358.png)
IntroductionCalcium hydroxide (Ca[OH]2) microcapsules were synthesized for use in controlled release. The aim of this study was to evaluate the cytotoxicity, antibacterial properties, and influence on gene expression of bone-related markers of 2 different formulas of Ca(OH)2 microcapsules.MethodsTwo formulas of Ca(OH)2 microcapsules (A and B) were evaluated, and pure Ca(OH)2 powder was used as a positive control. The shell material of formula A was pure EC, and the PLA/EC blend of 1:1 was used as the shell material for formula B. The MG63 cells/Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) were used to evaluate the cytotoxicity, and the colony-forming units of Enterococcus faecalis were monitored for the antibacterial effect. The relative messenger RNA expression of collagen I and osteocalcin was determined by real-time polymerase chain reaction.ResultsBoth formulas of the Ca(OH)2 microcapsules showed no cytotoxicity in MG63 cells; however, the Ca(OH)2 positive control did exhibit cytotoxicity. The antibacterial effect of the 2 microcapsule formulas lasted longer than the positive control, and formula A lasted longer than formula B. For both Ca(OH)2 microcapsule formulas, the relative messenger RNA expression of collagen I and osteocalcin was prolonged and up-regulated. The time effects of the influence on messenger RNA expression of collagen I and osteocalcin were different between the 2 microcapsule formulas.ConclusionsCa(OH)2 microcapsules had prolonged antibacterial activity and prolonged the up-regulation of bone-related markers with reduced cytotoxicity.
Journal: Journal of Endodontics - Volume 39, Issue 8, August 2013, Pages 1030–1034