کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3154479 1198049 2009 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs
چکیده انگلیسی

PurposeCurrent strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model.Materials and MethodsTooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses.ResultsSmall tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues.ConclusionsThis pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Oral and Maxillofacial Surgery - Volume 67, Issue 2, February 2009, Pages 335–347
نویسندگان
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