Compressive force induces prostaglandin E2 production via cyclooxygenase-2 in synovial cell

ObjectivesProstaglandin E2 (PGE2) and inflammatory cytokines play pivotal roles in the pathogenesis of temporomandibular joint (TMJ) diseases. These mediators are influenced by mechanical loading. To clarify a reaction to mechanical stress of synovial cells, we examined the effects of compressive force on levels of expression of PGE2 and cyclooxygenase (COX).Materials and methodsRabbit synovial lineage cell (HIG-82) were continuously compressed using a conventional method. PGE2 release in conditioned medium was monitored by enzyme linked immunosorbent assay. The expression of COX mRNA in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction.ResultsCompressive force was found to enhanced the production of PGE2 and mRNA expression of COX-2 in synovial cells. Further, indomethacin blocked compressive force-mediated production of PGE2.ConclusionsOur results indicated that the compressed force enhanced PGE2 production through COX-2 expression in synovial cells.