A comparative analysis of root surface biomodification with ethylene diamine tetra acetic acid and tetracycline hydrochloride: An in vitro scanning electron microscopic study

BackgroundThe outcome of periodontal regenerative therapy depends upon the of the biocompatibility root surfaces to the regenerating periodontal tissues. This in vitro scanning electron microscopic (SEM) study was designed to evaluate and compare the demineralizing efficacy of ethylene diamine tetra acetic acid (EDTA), with that of tetracycline hydrochloride applied on to the mechanically treated root surfaces of periodontally involved tooth.MethodsForty specimens were prepared from teeth extracted due to advanced periodontal disease and divided into two groups. The study group was treated with an EDTA solution (pH 7.4) and the control group was treated with a tetracycline hydrochloride solution (pH 1.8). The photomicrographs obtained were assessed for presence of smear layer, number of exposed dentinal tubules, area occupied by tubule orifices along with intertubular surface appearance. The results thus obtained were analyzed statistically.ResultsBoth EDTA and tetracycline were effective in removing the smear layer and the exposure of the number of dentinal tubules. The diameters of the tubules and thereby the surface area occupied by the tubule orifices in the EDTA treated group were significantly greater than the tetracycline HCL treated group (p < 0.05).ConclusionThe EDTA produced better effects than tetracycline by providing more demineralized area and collagen exposure at a neutral pH.