کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3214160 | 1203272 | 2008 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes](/preview/png/3214160.png)
SummaryBackgroundIonizing radiation is used to treat a lot of cancers, however, it also produced unwanted side effect on normal tissues, such as radiodermatitis. We previously established an animal model for radiodermatitis, and identified many of radiation-induced genes by cDNA microarray. Of the candidates, we chose S100A8 gene for a further study.ObjectiveThe aim of this study is to investigate the functional role of S100A8 in X-ray irradiated keratinocytes.MethodsRT-PCR and immunohistochemistry were performed to demonstrate the S100A8 induction by X-ray irradiation. HaCaT keratinocytes were transduced with the recombinant adenovirus expressing GFP-S100A8, and then effects on cell cycle and apoptosis were analyzed using flow cytometry and Western blot.ResultsX-ray irradiation markedly induced S100A8 expression in the hyperplastic epidermis of mouse. Overexpression of S100A8 by adenoviral transduction led to the enhancement of cell proliferation in the absence and/or presence of X-ray irradiation, as compared with Ad/GFP control group. Furthermore, overexpression of S100A8 significantly protected the X-ray-induced apoptosis.ConclusionThese results suggest that S100A8 have an anti-apoptotic role in X-ray irradiated keratinocytes.
Journal: Journal of Dermatological Science - Volume 51, Issue 1, July 2008, Pages 11–18