کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3214502 1203298 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of keratinocyte apoptosis by photosensitizing chemicals plus UVA
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی امراض پوستی
پیش نمایش صفحه اول مقاله
Induction of keratinocyte apoptosis by photosensitizing chemicals plus UVA
چکیده انگلیسی

SummaryBackgroundThe capacity of photosensitizing chemicals with ultraviolet A light (UVA) to induce apoptosis is one of the methods to assess their phototoxic and potentially photoallergic properties, since apoptotic cells may be easily presented by antigen-presenting cells.ObjectivesWe examined the photoaggravated ability to induce keratinocyte apoptosis of various chemicals that are known as causative agents of photocontact dermatitis and drug photosensitivity involving photoallergic and/or phototoxic mechanisms.MethodsHaCaT keratinocytes were incubated with 3,3′,4′,5-tetrachlorosalicylanilide (TCSA), bithionol, diphenylhydramine, chlorpromazine, 6-methylcoumarin, sparfloxacin, and enoxacin at 10−7 to 10−4 M and irradiated with UVA at 4 J/cm2. As positive control, 8-methoxypsoralen (8-MOP) was also tested. Apoptosis and necrosis were evaluated by flow cytometric enumeration of annexin V+ 7-AAD− and annexin V+ 7-AAD+ cells, respectively. The expression of apoptosis-related molecules, caspase-3 and poly (ADP-ribose) polymerase (PARP), was tested by flow cytometric and Western blotting analyses.ResultsIn a comparison with non-irradiated cells, significant apoptosis was found in TCSA, bithionol, chlorpromazine, sparfloxacin and enoxacin at 10−4 or 10−5 M as well as 8-MOP as assessed by both annexin V and active caspase-3 stainings, while necrosis occurred in most of these chemicals at 10−4 M. Neither apoptosis nor necrosis was seen in diphenylhydramine or 6-methylcoumarin. PARP were activated in HaCaT cells phototreated with TCSA, bithionol and chlorpromazine.ConclusionsWe suggest that our method is useful for in vitro assessment of phototoxicity and potential photoallergenicity of chemicals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dermatological Science - Volume 45, Issue 2, February 2007, Pages 105–112
نویسندگان
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