کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3265188 1207809 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of Wnt-5a and its clinicopathological significance in hepatocellular carcinoma
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی غدد درون ریز، دیابت و متابولیسم
پیش نمایش صفحه اول مقاله
Expression of Wnt-5a and its clinicopathological significance in hepatocellular carcinoma
چکیده انگلیسی

BackgroundThe functions of Wnt-5a in human cancers are controversial and unclear.AimTo investigate the clinical significance of Wnt-5a expression in hepatocellular carcinoma.Patients and methods Real-time quantitative Reverse transcriptase Polymerase Chain Reaction was done to evaluate Wnt-5a gene expression. Wnt-5a, β-catenin, E-cadherin and Ki-67 were examined immunohistochemically in 114 hepatocellular carcinoma cases.ResultsCompared to normal tissue, Wnt-5a mRNA expression was clearly increased in hepatocellular carcinoma, chronic hepatitis and cirrhosis. On immunohistochemistry, immunostaining of Wnt-5a showed a bell-shaped pattern: low to undetectable levels were present in normal tissue and in tumour samples, whereas strong immunostaining was seen in chronic hepatitis, cirrhosis and dysplastic liver cells. Reduction or loss of Wnt-5a protein expression was found in 80.7% of hepatocellular carcinoma cases (n = 92) and was significantly associated with higher tumour stage (p < 0.001), serum AFP level (p = 0.025), low membranous expression of E-cadherin (p < 0.0001) and β-catenin (p = 0.036) and high Ki-67 labelling indices (LIs, p = 0.001).ConclusionWnt-5a mRNA and protein levels are higher than normal in hepatitis and cirrhosis and appear to be related to the presence of hepatitis B virus infection. However, Wnt-5a protein expression is frequently lost in hepatocellular carcinoma; this supports the notion that this protein has a tumour suppressor function in hepatocellular carcinoma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Digestive and Liver Disease - Volume 40, Issue 7, July 2008, Pages 560–567
نویسندگان
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