کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3266158 1207834 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Increased prevalence of celiac disease without gastrointestinal symptoms in adults MICA 5.1 homozygous subjects from the Campania area
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی غدد درون ریز، دیابت و متابولیسم
پیش نمایش صفحه اول مقاله
Increased prevalence of celiac disease without gastrointestinal symptoms in adults MICA 5.1 homozygous subjects from the Campania area
چکیده انگلیسی

ObjectivesPolymorphisms in the major histocompatibility complex class I chain-related gene A may influence its binding to the Natural Killer Cell Receptor G2D (NKG2D). We looked for polymorphisms in major histocompatibility complex class I chain-related gene A exon 5 and in Human Leukocyte Antigen (HLA)-DQ/DR in adult coeliac disease patients to determine whether they affected coeliac disease phenotypes.MethodsAdult coeliac disease patients with (n = 98) and without (n = 93) gastrointestinal symptoms (gastrointestinal symptoms+/gastrointestinal symptoms−) and 108 control subjects from Campania (Italy) were characterized by Polymerase Chain Reaction (PCR) sequence specific oligonucleotide followed by PCR sequence specific primer assays for HLA DQ/DR, and by PCR followed by capillary electrophoresis for major histocompatibility complex class I chain-related gene A exon 5 polymorphisms. Immunoglobulin A (IgA) anti-transglutaminase antibodies were also evaluated by immunosorbent assay.ResultsFive different major histocompatibility complex class I chain-related gene A alleles were detected in both coeliac disease patients and control subjects. The major histocompatibility complex class I chain-related gene A 5.1 allele occurred more frequently in patients than in controls (p < 0.05), and the major histocompatibility complex class I chain-related gene A 5.1/5.1 homozygous genotype increased the risk of gastrointestinal symptoms− coeliac disease (OR = 2.79, 95% CI 1.15–6.79). Gastrointestinal symptoms− coeliac disease patients bearing major histocompatibility complex class I chain-related gene A 5.1/5.1 alleles showed lower anti-transglutaminase levels (18 U/L) than the gastrointestinal symptoms+ coeliac disease patients (35 U/L). HLA-DQ2/DQ8 genotypes did not differ between gastrointestinal symptoms+ and gastrointestinal symptoms− coeliac disease, although DQ8 tended to be more frequent in gastrointestinal symptoms− coeliac disease (11.7%) than in gastrointestinal symptoms+ coeliac disease (6%).ConclusionsOur study shows that a double dose of the major histocompatibility complex class I chain-related gene A 5.1 allele could predispose to the onset of gastrointestinal symptoms− coeliac disease. We can hypothesize that a lower level of immunological involvement in gastrointestinal symptoms− coeliac disease patients is associated with absence of gastrointestinal symptoms. This test could represent a second step in the genetic typing of high-risk subjects such as first-degree relatives of coeliac disease patients positive for the DQ2/DQ8 molecule.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Digestive and Liver Disease - Volume 40, Issue 4, April 2008, Pages 248–252
نویسندگان
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