کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3277779 | 1208636 | 2006 | 9 صفحه PDF | دانلود رایگان |

ObjectiveThis study evaluated whether ω-3 polyunsaturated fatty acids (PUFAs) could enhance the radiosensitivity of three different human colorectal adenocarcinoma cell lines. To understand the underlying mechanisms, the effects of ω-3 PUFAs on the cell growth, survival, and apoptosis were evaluated alone or in combination with an antioxidant (vitamin E) and compared with the effects of ω-6 PUFAs.MethodsLS174T, CO112, and Caco-2 cell survival was assessed by clonogenic assay after a 3-d pretreatment with ω-3/ω-6 PUFAs and/or vitamin E before a single X-ray exposure to 4 Gy. Cell growth and viability were measured by double fluorescence-activated cell sorter analyses using propidium iodide and fluorescein isothiocyanate-conjugated annexin V. Student’s t test or multivariable linear regression analyses were used for comparison.ResultsPreincubation with 30 to 100 μmol/L of ω-3 PUFAs induced a dose-dependent additive decrease in cell survival after irradiation (P < 0.05). Evaluation of the underlying mechanisms indicated that ω-3 PUFAs mainly decreased the cell number via apoptosis induction. Moreover, formation of lipid peroxidation products and modulation of cyclooxygenase II activity seemed to be involved, because coincubation with 10 μmol/L vitamin E abolished the effect of 50 μmol/L of ω-3 PUFAs (P < 0.05), whereas ω-6 PUFAs could partly mimic ω-3 PUFA effects.ConclusionThese observations suggest that ω-3 PUFAs may be potential candidates as nutritional adjuvants to enhance the efficacy of human colorectal cancer radiotherapy.
Journal: Nutrition - Volume 22, Issue 9, September 2006, Pages 931–939