کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3299067 | 1209921 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transient Receptor Potential Vanilloid 1 Mediates Hyperalgesia and Is Up-Regulated in Rats With Chronic Pancreatitis
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کلمات کلیدی
TRPV1VFFTNBSNGFRT-PCRCTBLCMDRGdorsal root ganglion - گانگلیون ریشه پشتیDiI - DIIvon Frey filament - از رشته فرایTrinitrobenzene sulfonic acid - اسید سولفونیک Trinitrobenzenecholera toxin B - تومور مثانه Bnerve growth factor - فاکتور رشد عصبlaser capture microdissection - لیزر ضبط میکرو دیسکسیونreverse-transcription polymerase chain reaction - واکنش زنجیره پلیمراز معکوس رونویسیchronic pancreatitis - پانکراتیت مزمنtransient receptor potential vanilloid receptor 1 - پتانسیل گیرنده گذرا گیرنده وانیلوئید 1CaP - کلاه لبه دارCapsaicin - کپسایسین یا کاپسیسین
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transient Receptor Potential Vanilloid 1 Mediates Hyperalgesia and Is Up-Regulated in Rats With Chronic Pancreatitis Transient Receptor Potential Vanilloid 1 Mediates Hyperalgesia and Is Up-Regulated in Rats With Chronic Pancreatitis](/preview/png/3299067.png)
چکیده انگلیسی
Background & Aims: The neurobiologic basis of pancreatic hyperalgesia in chronic pancreatitis (CP) is understood poorly and there is a need to identify novel therapeutic targets. Our aim was to study the role of the transient receptor potential vanilloid 1 (TRPV1), a key integrator of noxious stimuli, in the pathogenesis of pancreatic pain in a rat model of CP. Methods: CP was induced in rats by intraductal injection of trinitrobenzene sulfonic acid. TRPV1 currents in pancreas-specific DRG neurons were measured using perforated patch-clamp techniques. Reverse-transcription polymerase chain reaction was used to measure mRNA expression of TRPV1 in these neurons after laser capture microdissection. Immunofluorescence and Western blot analysis, using TRPV1-specific antibodies, also were performed. Pancreatic hyperalgesia was assessed by rat's nocifensive behavior to electrical stimulation of the pancreas. Results: CP was associated with a 4-fold increase in capsaicin-induced current density (P < .02), along with an increase in the proportion of pancreas-specific DRG neurons that responded to capsaicin (52.9% in controls vs 79.0% in CP; P < .05). CP also was associated with a significant increase in TRPV1 expression both at the messenger RNA and protein level in whole thoracic DRGs and pancreas-specific sensory neurons. Systemic administration of the TRPV1 antagonist SB-366791 markedly reduced both visceral pain behavior and referred somatic hyperalgesia in rats with CP, but not in control animals. Conclusions: TRPV1 up-regulation and sensitization is a specific molecular mechanism contributing to hyperalgesia in CP and represents a useful target for treating pancreatic hyperalgesia caused by inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 133, Issue 4, October 2007, Pages 1282-1292
Journal: Gastroenterology - Volume 133, Issue 4, October 2007, Pages 1282-1292
نویسندگان
Guang-Yin Xu, John H. Winston, Mohan Shenoy, Huaizhi Yin, Swaroop Pendyala, Pankaj Jay Pasricha,