کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3299154 | 1209923 | 2008 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Murine Embryonic Stem Cell-Derived Pancreatic Acinar Cells Recapitulate Features of Early Pancreatic Differentiation
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کلمات کلیدی
CPAqRT-PCRCCKCarboxypeptidase Aα-Amylase - α-آمیلازAdenovirus - آدنوویروسamyl - آمیلembryoid bodies - جنین های جنینیembryonic stem - ساقه جنینConditioned medium - شرایط محیطیquantitative reverse-transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی معکوس رونویسیcholecystokinin - کولهسیستوکینینZymogen granules - گرانول Zymogen
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: Acinar cells constitute 90% of the pancreas epithelium, are polarized, and secrete digestive enzymes. These cells play a crucial role in pancreatitis and pancreatic cancer. However, there are limited models to study normal acinar cell differentiation in vitro. The aim of this work was to generate and characterize purified populations of pancreatic acinar cells from embryonic stem (ES) cells. Methods: Reporter ES cells (Ela-pur) were generated that stably expressed both β-galactosidase and puromycin resistance genes under the control of the elastase I promoter. Directed differentiation was achieved by incubation with conditioned media of cultured fetal pancreatic rudiments and adenoviral-mediated co-expression of p48/Ptf1a and Mist1, 2 basic helix-loop-helix transcription factors crucial for normal pancreatic acinar development and differentiation. Results: Selected cells expressed multiple markers of acinar cells, including digestive enzymes and proteins of the secretory pathway, indicating activation of a coordinated differentiation program. The genes coding for digestive enzymes were not regulated as a single module, thus recapitulating what occurs during in vivo pancreatic development. The generated cells displayed transient agonist-induced Ca2+ mobilization and showed a typical response to physiologic concentrations of secretagogues, including enzyme synthesis and secretion. Importantly, these effects did not imply the acquisition of a mixed acinar-ductal phenotype. Conclusions: These studies allow the generation of almost pure acinar-like cells from ES cells, providing a normal cell-based model for the study of the acinar differentiation program in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 135, Issue 4, October 2008, Pages 1301-1310.e5
Journal: Gastroenterology - Volume 135, Issue 4, October 2008, Pages 1301-1310.e5
نویسندگان
Meritxell Rovira, Fabien Delaspre, Mohammad Massumi, Selma A. Serra, Miguel Angel Valverde, Josep Lloreta, Marlène Dufresne, Bruno Payré, Stephen F. Konieczny, Pierre Savatier, Francisco X. Real, Anouchka Skoudy,