کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3299309 | 1209926 | 2008 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Raf Protects Against Colitis by Promoting Mouse Colon Epithelial Cell Survival Through NF-κB
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کلمات کلیدی
NF-κBSDSDSSInhibitor of nuclear factor-κBERKIκBIKKYAMCMAPK - MAPKSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAinterleukin - اینترلوکینInhibitor of κB kinase - بازدارنده kB kinaseTUNEL - تونلyoung adult mouse colon - جوان کولون موش بالغsodium dodecyl sulfate - سدیم دودسیل سولفاتDextran sulfate sodium - سولفات سدیم دکسترانextracellular signal regulated kinase - سیگنال خارج سلولی kinase را تنظیم می کندnuclear factor-κB - فاکتور هسته ای κBmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال است
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Background & Aims: Raf-1 kinase is a key regulator of a number of cellular processes, which promote the maintenance of a healthy colon epithelium. This study addresses the role of Raf in epithelial cell survival in response to dextran sulfate sodium (DSS)-induced injury and inflammation. Methods: Inducible intestinal epithelium-specific Raf knockout mice were generated and subjected to acute colitis followed by a short recovery period. Colon sections were analyzed by in situ oligo ligation or immunostaining for Ki67, phospho-extracellular signal regulated kinase, and nuclear factor-κB p65. Western blot analysis and terminal deoxynucleotidyl transferase nick-end labeling assays were performed on Raf small interfering RNA-transfected young adult mouse colon cells following DSS treatment. Results: We report that Raf protects against epithelial injury and inflammation and promotes recovery from acute DSS-induced colitis by both MAPK/ERK kinase (MEK)-dependent and -independent pathways. Furthermore, we demonstrate that Raf induces novel cell survival responses through activating nuclear factor-κB in a MEK-independent manner. Conclusions: These novel findings indicate a protective role for Raf in colon epithelium following ulcerative damage through inhibiting cell apoptosis and promoting proliferation with important implications for responses such as inflammation-associated carcinogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 135, Issue 2, August 2008, Pages 539-551.e3
Journal: Gastroenterology - Volume 135, Issue 2, August 2008, Pages 539-551.e3
نویسندگان
Karen L. Edelblum, M. Kay Washington, Tatsuki Koyama, Sylvie Robine, Manuela Baccarini, D. Brent Polk,