کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
331148 1433645 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A non-toxic ligand for voxel-based MRI analysis of plaques in AD transgenic mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
A non-toxic ligand for voxel-based MRI analysis of plaques in AD transgenic mice
چکیده انگلیسی

Amyloid plaques are a characteristic feature in Alzheimer's disease (AD). A novel non-toxic contrast agent is presented, Gd-DTPA-K6Aβ1–30, which is homologous to Aβ, and allows plaque detection in vivo. μMRI was performed on AD model mice and controls prior to and following intracarotid injection with Gd-DTPA-K6Aβ1–30 in mannitol solution, to transiently open the blood–brain barrier. A gradient echo T2*-weighted sequence was used to provide 100 μm isotropic resolution with imaging times of 115 min. The scans were examined with voxel-based analysis (VBA) using statistical parametric mapping, for un-biased quantitative comparison of ligand-injected mice and controls. The results indicate that: (1) Gd-DTPA-K6Aβ1–30 is an effective, non-toxic, ligand for plaque detection when combined with VBA (p ≤ 0.01–0.001), comparing pre and post-ligand injection scans. (2) Large plaques can be detected without the use of a contrast agent and this detection co-localizes with iron deposition. (3) Smaller, earlier plaques require contrast ligand for MRI visualization. Our ligand when combined with VBA may be useful for following therapeutic approaches targeting amyloid in transgenic mouse models.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Aging - Volume 29, Issue 6, June 2008, Pages 836–847
نویسندگان
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