کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
331271 1433646 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Platelet β-secretase activity is increased in Alzheimer's disease
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Platelet β-secretase activity is increased in Alzheimer's disease
چکیده انگلیسی

β-Secretase activity is the rate-limiting step in Aβ peptide production from amyloid precursor protein. Aβ is a major component of Alzheimer's disease (AD) cortical amyloid plaques. β-Secretase activity is elevated in post mortem brain tissue in AD. The current study investigated whether β-secretase activity was also elevated in peripheral blood platelets. We developed a novel fluorimetric β-secretase activity assay to investigate platelets isolated from individuals with AD (n = 86), and age-matched controls (n = 115). Platelet membrane β-secretase activity (expressed as initial rate) varied over fourfold between individuals, raising important questions about in vivo regulation of this proteolytic activity. Nonetheless, we identified a significant 17% increase in platelet membrane β-secretase activity in individuals with AD compared to controls (p = 0.0003, unpaired t-test). Platelet membrane β-secretase activity did not correlate with mini-mental state examination (MMSE) score in the AD group (mean MMSE = 17.7, range 1–23), indicating that the increase did not occur as a secondary result of the disease process, and may even have preceded symptom onset.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Aging - Volume 29, Issue 5, May 2008, Pages 661–668
نویسندگان
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