کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3318346 | 1590360 | 2009 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pancreatitis-Associated Protein Inhibits Human Pancreatic Stellate Cell MMP-1 and -2, TIMP-1 and -2 Secretion and RECK Expression
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pancreatitis-Associated Protein Inhibits Human Pancreatic Stellate Cell MMP-1 and -2, TIMP-1 and -2 Secretion and RECK Expression Pancreatitis-Associated Protein Inhibits Human Pancreatic Stellate Cell MMP-1 and -2, TIMP-1 and -2 Secretion and RECK Expression](/preview/png/3318346.png)
چکیده انگلیسی
Background/Aims: Pancreatic stellate cells (PSCs) play a key role in fibrogenesis associated with acute and chronic pancreatitis. Pancreatitis-associated protein (PAP), an acute-phase protein, is dramatically upregulated during acute and chronic pancreatitis. Assuming a protective role of PAP, we investigated its effects on human PSCs. Methods: PSCs were obtained by outgrowth from fibrotic human pancreastissue. PAP was expressed in the yeast Pichia pastoris. PAP was added at 10 ng/ml to cultured PSCs. Cell proliferation was determined by bromodeoxyuridine incorporation. PSC migration was assessed by a wound healing assay. Collagen types I and III, fibronectin, matrix metalloproteinases (MMPs), tissue inhibitors of MMPs (TIMPs) and reversion-inducing cysteinerich protein with Kazal motifs (RECK) were demonstrated on protein and mRNA level. Results: PAP had no significant effect on PSC proliferation and migration. Cell-associated fibrillar collagen types I and III and fibronectin increased after addition of PAP to PSCs. PAP diminished the expression of MMP-1 and -2 and TIMP-1 and -2 and their concentrations in PSC supernatants. RECK was detected on the surface of PSCs and its expression was reduced after PAP application. Conclusions: Our data offer new insights into the biological functions of PAP, which may play an important role in wound healing response and cell-matrix interactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pancreatology - Volume 9, Issues 1â2, 2009, Pages 99-110
Journal: Pancreatology - Volume 9, Issues 1â2, 2009, Pages 99-110
نویسندگان
Ling Li, Max G. Bachem, Shaoxia Zhou, Zilin Sun, Jinfei Chen, Marco Siech, Daniel Bimmler, Rolf Graf,