کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3343697 1591183 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of resistance acquisition during tuberculosis treatment using whole genome sequencing
ترجمه فارسی عنوان
بررسی کسب مقاومت در طول درمان سل با استفاده از طیف تعیین توالی ژنوم
کلمات کلیدی
بیماری سل؛ آزمون حساسیت به مواد مخدر، تعیین توالی ژنوم مجموع
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی

Tuberculosis (TB) is still considered a major global public health problem in the world and there is a concern about the worldwide increase of drug-resistance (DR). This paper describes the analysis of three Mycobacterium tuberculosis isolates from a single patient collected over a long treatment period of time. DR was initially investigated through phenotypic testing, followed by line probe assays (LPAs) and whole genome sequencing (WGS). It presents an intriguing situation where a multidrug-resistant (MDR-) TB case was diagnosed and treated based only on late phenotypic drug susceptibility testing of isolate 1. During the treatment, another two isolates were cultivated: isolate 2, nine months after starting MDR-TB treatment; and isolate 3, cultivated five months later, during regular use of anti-TB drugs. These two isolates were evaluated using molecular LPA and WGS, retrospectively. All mutations detected by LPA were also detected in the WGS, including conversion from fluoroquinolones susceptibility to resistance from isolate 2 to isolate 3. WGS showed additional mutations, including some which may confer resistance to other drugs not tested (terizidone/cycloserine) and mutations with no correspondent resistance in drug susceptibility testing (streptomycin and second-line injectable drugs).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Brazilian Journal of Infectious Diseases - Volume 20, Issue 3, May–June 2016, Pages 290–293
نویسندگان
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