کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3344130 1591195 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
چکیده انگلیسی

ObjectivesEnterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.MethodsIn the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and Western blotting. Efficiency of the immune sera against ETEC was evaluated.ResultsAntibody induction was followed by immunization of mice with the chimeric protein. Pretreatment of the ETEC cells with immunized animal antisera remarkably decreased their adhesion to Caco-2 cells.DiscussionThe results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Brazilian Journal of Infectious Diseases - Volume 18, Issue 3, May–June 2014, Pages 308–314
نویسندگان
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