کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3347272 | 1215956 | 2012 | 5 صفحه PDF | دانلود رایگان |
Emergence of polymyxin resistance in carbapenem-resistant isolates is a great concern in clinical settings because it may mean the end of treatment options against Gram-negative bacterial infections. Polymyxin-nonsusceptible and -susceptible Pseudomonas aeruginosa isolates resistant to carbapenems and harboring blaIMP-6 were alternatively isolated from a patient. In vitro antimicrobial susceptibility testing, multilocus sequence typing, and pulsed-field gel electrophoresis were performed. Metallo-β-lactamase genes such as blaIMP, blaVIM, blaSPM, blaGIM, and blaSIM and blaOXA-50 were detected by polymerase chain reaction. Sequences of 2-component systems, PmrAB and PhoPQ, were also determined. All showed ST235 and the same pulsotype. Amino acid substitutions were identified in PmrB and PhoP from polymyxin-nonsusceptible isolates. Colistin exposure might be associated with the recovery of polymyxin-nonsusceptible isolates in this patient.
Journal: Diagnostic Microbiology and Infectious Disease - Volume 72, Issue 3, March 2012, Pages 267–271