کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3348240 | 1216004 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and sequencing of the class A β-lactamase gene (blaTEM-15) located on a chromosomal Tn801 transposon
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
Escherichia coli CA0210 was identified in a stool culture of a 03-mounth-newonate in Tunisia. This strain was resistant to β-lactams, including ureidopenicillins, ticarcillin–clavulanic acid, cefpirome, ceftazidime, and cefotaxime, but it remained susceptible to imipenem and cefoxitin. The β-lactam–hydrolyzing β-lactamase gene of E. coli CA0210 and the upstream and downstream regions were cloned, sequenced, and expressed in E. coli DH5α. These resistances were carried by a 1080-bp chromosomal gene that encoded a β-lactamase with a pI of 6.3. Cloning and sequencing experiments showed that the corresponding blaTEM-15 gene was part of a chromosomally located Tn801 transposon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 58, Issue 4, August 2007, Pages 459–463
Journal: Diagnostic Microbiology and Infectious Disease - Volume 58, Issue 4, August 2007, Pages 459–463
نویسندگان
Chedly Chouchani, Nahed Ben-Achour, Arij M'Charek, Omrane Belhadj,