کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3348458 | 1216014 | 2006 | 6 صفحه PDF | دانلود رایگان |
Despite limitations of swab transport systems (STS), many clinical samples and bacterial isolates are sent on these devices. We evaluated the performance of 4 commercial STS: M40 Transystem™ (S1, Copan Italia, Bovezzo, Italy), UNI-TER™ (S2, MEUS, Piove di Sacco, Italy), Euromed™ (S3, LAB SERVICE, Surbo, Italy), and Eurotubo™ (S4, Deltalab, Rubí, Spain). Survival of ATCC and clinical strains with characterized resistance mechanisms, stored at room temperature and using the roll-plate method (NCCLS M40-A) after holding times of 0, 6, 24, 48, and 72 h, and 7 days, was assessed. After 24 h, only S1 was able to maintain Streptococcus pneumoniae (ATCC 6305), Haemophilus influenzae (10211), and Neisseria meningitidis (13090) viability with a percentage range of recovery with respect to the baseline count at zero time of 104–184%. Neisseria gonorrhoeae (43069) was uncultivable after 6 h with all STS except S1 (46% recovery). Eighteen percent recovery after 24 h and 7% after 6 h was observed for Fusobacterium nucleatum (25586) and Peptostreptococcus anaerobius (27337), respectively, but only with S1. Resistance mechanisms in clinical isolates do not affect survival with any of the STS.
Journal: Diagnostic Microbiology and Infectious Disease - Volume 56, Issue 1, September 2006, Pages 19–24