کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3350774 1216406 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of interleukin-15 on human CD3−CD117+CD56−OX40L+ cell differentiation
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Effects of interleukin-15 on human CD3−CD117+CD56−OX40L+ cell differentiation
چکیده انگلیسی

Lymphoid tissue inducer (LTi) cells are essential for secondary lymphoid tissue development, and recently identified human LTi cells are closely related to natural killer (NK) cells. In this study, we investigate whether human CD3−CD117+CD56− cells that include LTi and immature NK cells respond to interleukin (IL)–15, which is an NK cell growth factor. In the presence of IL-15, CD3−CD117+CD56− cells proliferate and downregulate the expression of OX40L and mRNA for IL-22, lymphotoxin-α, and aryl hydrocarbon receptor, but not Id2. To examine whether CD−CD117+CD56− cells differentiate into CD3−CD117+CD56+ NK cells by IL-15, we sorted CD3−CD117+CD56−OX40L+ cells and cultured with IL-15 for 7 days. Approximately 75% of the cells differentiated into imterferon-γ–expressing CD56+ cells and ∼25% of the cells did not. In addition, the latter population expressed LTi markers, including lymphotoxin-α and retinoid-related orphan receptor–γ (RORC). These results show that ∼25% of CD3−CD117+CD56−OX40L+ cells are LTi cells and do not differentiate into CD56+ NK cells by IL-15.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 71, Issue 8, August 2010, Pages 745–750
نویسندگان
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