کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3351508 1216431 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two sequence dimorphisms of DPB1 define the immunodominant serologic epitopes of HLA-DP
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Two sequence dimorphisms of DPB1 define the immunodominant serologic epitopes of HLA-DP
چکیده انگلیسی

We describe two sets of epitopes present in HLA-DP molecules; they were identified with alloantibodies from clinical serum samples. Specificity was determined using fluorescent beads coated with single antigens and detected in a Luminex platform. Of the patients with anti–HLA class II antibodies, 18% had anti-DP antibodies; among these, 24 of 32 patients (75%) had antibodies against the dimorphic epitope sets described here. Residues 56-A and 56-E divide DPB1 alleles into two mutually exclusive and collectively exhaustive groups. These groups have distinctive dimorphic epitopes that are detected by antibodies. Epitope P-001, identified by 2 sera, is defined by residue 56-A of the DPB subunit. Epitope P-002, identified by 9 sera, is defined by residue 56-E. Interlocus DRB1/DPB1 reactivity is associated with P-002, which is found in DRB1–DR11 alleles. Residues at DPB1 85-87-EAV define the P-003 epitope, whereas P-004 is defined by 85–87-GPM. This dimorphism also divides DPB1 alleles into two mutually exclusive and collectively exhaustive groups. In this study, 12 patient sera identified DP-003, and 1 identified DP-004. Two dimorphic systems account largely for the serologic features of the DP molecules, and these specificities were found in most clinical samples with anti-DP activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 70, Issue 10, October 2009, Pages 836–843
نویسندگان
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