کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3352099 1591380 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct Determination of Single Nucleotide Polymorphism Haplotype of NFKBIL1 Promoter Polymorphism by DNA Conformation Analysis and Its Application to Association Study of Chronic Inflammatory Diseases
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Direct Determination of Single Nucleotide Polymorphism Haplotype of NFKBIL1 Promoter Polymorphism by DNA Conformation Analysis and Its Application to Association Study of Chronic Inflammatory Diseases
چکیده انگلیسی
We previously revealed that one of the human leukocyte antigen-linked susceptibility genes for Takayasu's arteritis (TA) was mapped between TNFA and MICB loci and that −63T allele of NFKBIL1, which is between TNFA and MICB loci, was associated with rheumatoid arthritis (RA) in the Japanese population. We have developed a novel typing method based on reference strand-mediated conformation analysis for the upstream sequence of the NFKBIL1 gene, where −422 (T)8/(T)9, −325 C/G, −263 A/G, and −63 T/A polymorphisms were found. Upon the analysis of the patients with TA (n = 84), those with RA (n = 120), and healthy control subjects (n = 217), five common haplotypes named IKBLp*01 through IKBLp*05 were found in the Japanese population. The frequency of IKBLp*03 was significantly increased in the patient with TA (57.1% vs 35.0%, giving an odds ratio of 2.47). In addition, the frequency of IKBLp*01, but not that of other −63T-bearing alleles, was increased in the patients with RA (73.3% vs 58.1%, giving an odds ratio of 1.99), suggesting that the susceptibility to RA was conferred not by −63T alone but by combination of single nucleotide polymorphisms in the NFKBIL1 promoter. A higher promoter activity associated with IKBLp*03 and a lower activity associated with IKBLp*01 may contribute to the susceptibility to TA and RA, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 67, Issues 4–5, April–May 2006, Pages 363-373
نویسندگان
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