کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3352272 1216468 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sequence Analysis of Major Histocompatibility Complex Class-II DQB1 (Patr-DQB1) Alleles in Chimpanzees by Polymerase Chain Reaction–Based Methods
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Sequence Analysis of Major Histocompatibility Complex Class-II DQB1 (Patr-DQB1) Alleles in Chimpanzees by Polymerase Chain Reaction–Based Methods
چکیده انگلیسی

The major histocompatibility complex (MHC) class-II genes are described as the genes that encode the antigen-presenting molecule. In particular, functional alleles of MHC class-II genes in nonhuman primates have been analyzed as part of the study of infectious and immune-mediated diseases in animal models and as part of efforts to understand the molecular evolution of human leukocyte antigen. The polymorphisms and sequence analysis of MHC class-II genes in a large number of subjects is necessary for the group management of nonhuman primates. In the present study, we attempted to analyze the DQB1 polymorphism in the common chimpanzee (Pan troglodytes) by exon 2 sequencing. For exact typing of Patr-DQB1 alleles, we carried out polymerase chain reaction–restriction fragment length polymorphism and sequence analysis. In the genomic DNA of 25 chimpanzees, 6 Patr-DQB1 alleles, including 2 new alleles, were detected. Identification and analysis of Patr-DQB1 alleles using this method may contribute to our understanding of Patr-DQB1 allelic diversity in individual chimpanzees and should be useful in facilitating colony management of chimpanzee groups in Japan.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 67, Issue 8, August 2006, Pages 655–663
نویسندگان
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