کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3352710 1216505 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interleukin-10 and Tumor Necrosis Factor–α Single Nucleotide Gene Polymorphism Frequency in Paracoccidioidomycosis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Interleukin-10 and Tumor Necrosis Factor–α Single Nucleotide Gene Polymorphism Frequency in Paracoccidioidomycosis
چکیده انگلیسی

Allelic variants of cytokine genes seem to be involved in mechanisms of resistance or susceptibility to several diseases. The aim of this study was to investigate the frequency of genotypes with the tumor necrosis factor–α TNF-α gene polymorphism G/A at position −308 and the IL-10 gene polymorphism G/A at position −1082, and to verify a possible association of these polymorphisms with paracoccidioidomycosis (PCM) caused by Paracoccidioides brasiliensis. Genotyping was performed by allele-specific polymerase chain reaction (ASPCR) and restriction fragment length polymorphism (RFLP) on genomic DNA isolated of granulocytes from 54 PCM patients and 31 noninfected individuals. The analysis of SNP at position −1082 IL-10 showed a high frequency of GA genotype in both patients and controls (51% and 55%, respectively), while the allelic frequency showed 54% of G allele in the patients and 66% of A allele in the controls. The GG genotype was more frequent in patients (85%) and controls (68%) when we analyze the SNP at position −308 of TNF-α gene. Otherwise, 91% of PCM patients and 84% of noninfected individuals carried the G allele in −308 TNF-α SNP. Stimulation of cells from individuals with PCM phenotyped as A+ (GA or AA genotypes) presented elevation of TNF-α producing cells when compared with IL-10–producer cells. These findings reinforce the critical role of IL-10 and TNF-α in the paracoccidioidomycosis and can strongly suggest that the genetic screening of the −308G/A and −1082G/A polymorphisms may be a valid tool for identification of subjects needing a more appropriate therapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 67, Issue 11, November 2006, Pages 931–939
نویسندگان
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