کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3358568 | 1591761 | 2015 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Assays for therapeutic drug monitoring of β-lactam antibiotics: A structured review Assays for therapeutic drug monitoring of β-lactam antibiotics: A structured review](/preview/png/3358568.png)
• Pharmacokinetics may be severely altered in special patient populations.
• Therapeutic drug monitoring might be useful to optimise dosing.
• No commercial assays are currently available.
• A systematic review was performed of all published methods.
In some patient groups, including critically ill patients, the pharmacokinetics of β-lactam antibiotics may be profoundly disturbed due to pathophysiological changes in distribution and elimination. Therapeutic drug monitoring (TDM) is a strategy that may help to optimise dosing. The aim of this review was to identify and analyse the published literature on the methods used for β-lactam quantification in TDM programmes. Sixteen reports described methods for the simultaneous determination of three or more β-lactam antibiotics in plasma/serum. Measurement of these antibiotics, due to low frequency of usage relative to some other tests, is generally limited to in-house chromatographic methods coupled to ultraviolet or mass spectrometric detection. Although many published methods state they are fit for TDM, they are inconvenient because of intensive sample preparation and/or long run times. Ideally, methods used for routine TDM should have a short turnaround time (fast run-time and fast sample preparation), a low limit of quantification and a sufficiently high upper limit of quantification. The published assays included a median of 6 analytes [interquartile range (IQR) 4–10], with meropenem and piperacillin being the most frequently measured β-lactam antibiotics. The median run time was 8 min (IQR 5.9–21.3 min). There is also a growing number of methods measuring free concentrations. An assay that measures antibiotics without any sample preparation would be the next step towards real-time monitoring; no such method is currently available.
Journal: International Journal of Antimicrobial Agents - Volume 46, Issue 4, October 2015, Pages 367–375