کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3361934 | 1592054 | 2015 | 8 صفحه PDF | دانلود رایگان |

• Oxacillinases correlated with 90% of multiple drug-resistant (MDR) Acinetobacter baumannii from a single hospital.
• GES-type carbapenemases and ISAba1 were also detected.
• Insertion elements are likely behind resistance via β-lactamase production.
• The majority of the strains belonged to sequence type 2.
• Genotyping of resistance aids in understanding MDR A. baumannii transmission.
SummaryObjectivesThe aim of this study was to identify the genetic relatedness of multiple drug-resistant (MDR) Acinetobacter baumannii clinical isolates recovered from a hospital in Los Angeles.MethodsTwenty-one MDR A. baumannii isolates were collected and their antibiotic susceptibilities determined according to Clinical and Laboratory Standards Institute guidelines. Genes coding for antibiotic resistance were identified by PCR, and their identities were confirmed by DNA sequencing. Clonal relationships were studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).ResultsMDR consistently correlated with the presence of oxacillinases, mostly in the form of the plasmid-mediated OXA-23 enzyme, which was detected in 12 (57.1%) isolates. GES-type carbapenemases were found in 20 (95.2%) strains, AAC in all 21 (100%) strains, and PER in seven (33.3%) strains, and ISAba1 was detected in 16 (76.2%) isolates. The association between ISAba1 and resistance genes confirms insertion elements as a source of β-lactamase production. Of the 21 clinical isolates, five were found to be related to sequence type 1 (ST1) and 16 to ST2, as analyzed by MLST. PFGE demonstrated that the majority of clinical isolates were highly related (>85%).ConclusionsThis study supports a more complete understanding of genotyping of antibiotic resistance for better assessment of MDR strain transmission.
Journal: International Journal of Infectious Diseases - Volume 41, December 2015, Pages 42–49