کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3362322 | 1592066 | 2014 | 7 صفحه PDF | دانلود رایگان |
• We compare qPCR and microscopy in the diagnosis of imported malaria in Serbia.
• qPCR was shown to be superior to microscopy in case of low parasitemia.
• qPCR was superior for species determination, particularly of non-falciparum species.
• Due to long DNA persistence, qPCR is not appropriate for monitoring drug effect.
SummaryObjectivesThe goal of this study was to assess the clinical significance of conventional and PCR-based molecular diagnosis in patients with imported malaria in Serbia.MethodsGiemsa microscopy, the rapid diagnostic test, and quantitative real-time PCR (qPCR) were used to detect Plasmodium species in 109 whole-blood samples from patients after their return from malaria endemic areas, including those clinically suspected for malaria (n = 97) and healthy travelers (n = 12) examined as part of epidemiological surveillance.ResultsA total of 45 patients were diagnosed with malaria: 42 (93.3%) by microscopy and three (6.7%) additional ones by qPCR. The agreement between the results of species-specific qPCR and microscopy was 73.3%; it was as high as 90.6% for Plasmodium falciparum infections. Follow-up analysis demonstrated persistence of Plasmodium sp DNA for a mean 6 days after the disappearance of parasitemia on microscopy.ConclusionsDue to its sensitivity and specificity, qPCR is a helpful method complementary to microscopy, particularly in cases of low parasitemia. In addition, it is superior to microscopy for species identification.
Journal: International Journal of Infectious Diseases - Volume 29, December 2014, Pages 24–30