کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3363186 | 1592100 | 2012 | 5 صفحه PDF | دانلود رایگان |

SummaryObjectivesThe aim of the present study was to identify and differentiate Mycobacterium kansasii and Mycobacterium chelonae–Mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries.MethodsPCR-restriction analysis of the hsp65 gene (PRA) with automated capillary electrophoresis was applied to the isolates previously identified by conventional biochemical testing and the molecular INNO-LiPA MYCOBACTERIA assay.ResultsPRA performed very well in comparison with the two other methods (96.4% concordance). Among 27 M. kansasii isolates, this method detected five genetic types, of which type 1 represented the most common clinical isolate, as it is worldwide. PRA differentiated 29 M. chelonae–M. abscessus group isolates into Mycobacterium immunogenum type 2 (n = 13), M. chelonae (n = 12), and M. abscessus types 1 (n = 1) and 2 (n = 1). M. immunogenum was the most frequent (69%) isolate from humans, but only one of 11 cases was clinically significant. M. chelonae was the most commonly (83%) recovered from water. PRA also identified two isolates with hsp65 alleles representing previously unreported patterns.ConclusionsPRA based on automated capillary electrophoresis is a rapid, simple, and reliable method for the identification and differentiation of both clinically relevant and environmental isolates of M. kansasii and M. chelonae–M. abscessus group.
Journal: International Journal of Infectious Diseases - Volume 16, Issue 3, March 2012, Pages e193–e197