کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3363481 1592105 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of biofilm formation on the excretion of Salmonella enterica serovar Typhi in feces
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Effect of biofilm formation on the excretion of Salmonella enterica serovar Typhi in feces
چکیده انگلیسی

SummaryObjectivesWe hypothesized that Salmonella enterica serovar Typhi (S. Typhi) with higher biofilm and capsule production capability are more able to survive continuously in typhoid patients/carriers, with subsequent prolonged shedding in feces.MethodsBacterial cell release from biofilm (produced in vitro and confirmed by specific staining and electron microscopy) and comparative cytotoxicity were studied on Caco2 cells. Functionality of the biofilm diffusion barrier was tested against ciprofloxacin. Biofilm production was graded and semi-quantified as −, +, ++, +++, and ++++.ResultsOut of 30 isolates, 23 produced biofilm. The average post-treatment detection of S. Typhi in blood was 7–13 days and in stool was 13–32 days. A fall in cell count from 10 4 to approximately 101 over the course of 3 days as compared to total elimination of planktonic cells in 16 h after ciprofloxacin application substantiated the protective role of biofilm. Lactic dehydrogenase release ranged from 38% in non-biofilm producers to 97% in the highest biofilm producers, indicating increased pathogenic behavior.ConclusionsThe period of S. Typhi clearance from typhoid patients after recovery was found to be directly related to biofilm production capability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Infectious Diseases - Volume 15, Issue 11, November 2011, Pages e747–e752
نویسندگان
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