کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3363943 1592108 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples
چکیده انگلیسی

SummaryObjectiveThis study was designed to evaluate the antigen capture enzyme immunoassays (EIAs) Triage parasite panel and TechLab Entamoeba histolytica II in detecting Giardia intestinalis, Cryptosporidium sp, and Entamoeba histolytica in fecal samples in comparison to microscopy, and in differentiating Entamoeba histolytica from Entamoeba dispar.MethodsThe Triage EIA was evaluated using 100 stool specimens that were tested by standard ova and parasite examination, including staining with both trichrome and modified acid-fast stains. Differentiation between E. histolytica and E. dispar was performed using TechLab.ResultsMicroscopic examination revealed that 19% of the samples were positive for Giardia, 4% for Cryptosporidium, and 1% for E. histolytica/E. dispar, and other parasites were found in 5%. By Triage, 23% of the samples were infected with Giardia, 5% with Cryptosporidium, and 2% with E. histolytica/E. dispar. Triage showed a sensitivity of 100% and specificity of 91.5%. The TechLab assay was negative for both samples diagnosed as E. histolytica/E. dispar by Triage, which suggested that they were E. dispar. Both tests showed no cross-reactivity with other intestinal protozoa.ConclusionThese results indicate that antigen detection by EIA has the potential to become a valuable tool, capable of making stool diagnostics more effective.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Infectious Diseases - Volume 15, Issue 8, August 2011, Pages e541–e544
نویسندگان
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