کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
337217 | 547467 | 2016 | 8 صفحه PDF | دانلود رایگان |
• Pre-conditioning ICV and LBNST antalarmin disrupts retention of contextual fear.
• Pre-conditioning ICV and LBNST antalarmin does not affect post-shock freezing.
• ICV and LBNST antalarmin does not affect unconditioned freezing to TMT predator odor.
• LBNST antalarmin does not affect responsivity to varying footshock intensities.
• LBNST CRFr1 receptors are important for long-term contextual fear learning and memory.
The bed nucleus of the stria terminalis (BNST) plays a critical role in fear and anxiety. The BNST is important for contextual fear learning, but the mechanisms regulating this function remain unclear. One candidate mechanism is corticotropin-releasing-factor (CRF) acting at CRF type 1 receptors (CRFr1s). Yet, there has been little progress in elucidating if CRFr1s in the BNST are involved in different types of fear (conditioned and/or unconditioned). Therefore, the present study investigated the effect of antalarmin, a potent CRFr1 receptor antagonist, injected intracerebroventricularly (ICV) and into the dorsolateral BNST (LBNST) during single trial contextual fear conditioning or exposure to the predator odor 2,5-dihydro-2,4,5-trimethylthiazoline (TMT). Neither ICV nor LBNST antalarmin disrupted unconditioned freezing to TMT. In contrast, ICV and LBNST antalarmin disrupted the retention of contextual fear when tested 24 h later. Neither ICV nor LBNST antalarmin affected baseline or post-shock freezing—indicating antalarmin does not interfere with the early phases of contextual fear acquisition. Antalarmin did not (1) permanently affect the ability to learn and express contextual fear, (2) change responsivity to footshocks, or (3) affect the ability to freeze. Our findings highlight an important role for CRFr1s within the LBNST during contextually conditioned fear, but not unconditioned predator odor fear.
Journal: Psychoneuroendocrinology - Volume 70, August 2016, Pages 17–24