کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3376648 1219918 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Meningococcal carriage rates in healthy individuals in Japan determined using Loop-Mediated Isothermal Amplification and oral throat wash specimens
ترجمه فارسی عنوان
میزان حمل و نقل مننژیکوکال در افراد سالم ژاپن تعیین شده با استفاده از روش تقویت ایزوترمال متصل شده به حلقه و نمونه های خوراکی شستشوی گلو
کلمات کلیدی
بیماری های مننژوکوک؛ ارتقاء ایزوترمال متصل به حلقه؛ حامل سالم؛ نمونه های خوراکی شستشوی گلو؛ Neisseria meningitidis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی

The detailed epidemiology of meningococcal diseases in Japan has yet to be determined and, moreover, the healthy carriage rate is also unknown. In this study, to obtain insight into the carriage rate of Neisseria meningitidis in healthy individuals in Japan, we developed a new method to detect the N. meningitidis-specific ctrB gene, one of the genes encoding enzymes for capsule synthesis, by Loop-Mediated Isothermal Amplification (LAMP) and examined the meningococcal carriage rate by using self-collected oral throat wash specimens from 836 students at a university. Examination by LAMP showed that 7 out of 836 samples were positive for N. meningitidis DNA, and the results were also verified by the nested PCR method for the meningococcus specific ggt gene. The N. meningitidis carriage rate in healthy individuals was estimated to be 0.84%. Moreover, we further confirmed by the nested-PCR-based serogroup typing method that 5 of the positive samples belonged to serogroup Y, 1 belonged to group B and 1 was unidentifiable. Considering the epidemiology for meningococcal diseases in Japan, the carriage rate and the serogroup profile seem to be consistent with low incidence of meningococcal diseases and serogroup distribution of clinical meningococcal isolates in Japan, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Infection and Chemotherapy - Volume 22, Issue 7, July 2016, Pages 501–504
نویسندگان
, , , , , , ,