کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3377846 1220054 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid identification of Mycobacterium avium clinical isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
ترجمه فارسی عنوان
شناسایی سریع جدایه های بالینی مایکوباکتریوم اوایم با ​​استفاده از اسپکترومتراسیون جریان زمانی پرواز دریافت کننده / یونیزاسیون ماتریس
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی

BackgroundRapid and accurate discrimination of Mycobacterium avium from other mycobacteria is essential for appropriate therapeutic management and timely intervention for infection control. However, routine clinical identification methods for M. avium are both time consuming and labor intensive. In the present study, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify specific cellular protein pattern for rapid identification of M. avium isolates.MethodsA total of 40 clinically relevant Mycobacterium strains comprising 13 distinct species were enrolled for the MALDI-TOF MS identification. A 10-minute extraction-free examination procedure was set up to obtain mass spectral fingerprints from whole bacterial cells.ResultsThe characteristic mass spectral peak patterns in the m/z (mass/charge ratio) range of 5–20 kDa can be obtained within 10 minutes. The species-specific mass spectra for M. avium is identified and can be differentiated from as Mycobacterium strains. This technique shortens and simplifies the identification procedure of MALDI-TOF MS and may further extend the mycobacterial MALDI-TOF MS database.ConclusionSimplicity and rapidity of identification procedures make MALDI-TOF MS an attractive platform in routine identification of mycobacteria. MALDI-TOF MS is applicable for rapid discrimination of M. avium from other Mycobacterium species, and shows its potential for clinical application.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiology, Immunology and Infection - Volume 48, Issue 2, April 2015, Pages 205–212
نویسندگان
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