کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3378355 1220079 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of CD14 gene promoter polymorphism in tuberculosis susceptibility
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
The role of CD14 gene promoter polymorphism in tuberculosis susceptibility
چکیده انگلیسی

BackgroundCD14 is expressed principally by cells of monocyte/macrophage lineage and plays a pivotal role in the innate immunity to intracellular infections. Recent research findings have revealed an association between the CD14 gene promoter polymorphism and several major infectious diseases.ObjectiveThe aim of the present study was to investigate the association between the CD14-159C/T polymorphism and tuberculosis in a Turkish population.MethodsFor this purpose, 88 consecutive patients with tuberculosis (63 pulmonary, 25 extrapulmonary) and 116 control subjects were enrolled into a prospective study. We determined CD14-159 genotypes by polymerase chain reaction - restriction fragment length polymorphism analysis and also measured serum concentrations of soluble CD14 (sCD14) by using a quantitative sandwich enzyme immunoassay technique.ResultsThere was no significant difference in terms of genotype distribution between patients with tuberculosis (CC 18.2%, CT 48.9%, TT 33.0%) and controls (CC 12.9%, CT 50.9%, TT 36.2%) or between patients with pulmonary and extrapulmonary tuberculosis. Serum levels of sCD14 were significantly increased in patients with active tuberculosis compared to those with inactive tuberculosis and healthy controls (p < 0.001). However, levels of sCD14 were not associated with any genotypes of CD14-159.ConclusionThe genotyping findings of the present study do not support a role for the CD14-159C/T polymorphism in the development of tuberculosis, at least in the geographical region of central Anatolia. Significantly elevated serum sCD14 levels in patients with active disease reflect the importance of the mononuclear phagocytic system activation in tuberculosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiology, Immunology and Infection - Volume 46, Issue 3, June 2013, Pages 158–163
نویسندگان
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