کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3379910 | 1220186 | 2012 | 7 صفحه PDF | دانلود رایگان |

SummaryObjectiveThe aim of this study was to demonstrate the potential of near-infrared (NIR) spectroscopy for categorizing cartilage degeneration induced in animal models.MethodThree models of osteoarthritic degeneration were induced in laboratory rats via one of the following methods: (1) menisectomy (MSX); (2) anterior cruciate ligament transection (ACLT); and (3) intra-articular injection of mono-ido-acetate (1 mg) (MIA), in the right knee joint, with 12 rats per model group. After 8 weeks, the animals were sacrificed and tibial knee joints were collected. A custom-made near-infrared (NIR) probe of diameter 5 mm was placed on the cartilage surface and spectral data were acquired from each specimen in the wave number range 4,000–12,500 cm−1. Following spectral data acquisition, the specimens were fixed and Safranin-O staining was performed to assess disease severity based on the Mankin scoring system. Using multivariate statistical analysis based on principal component analysis and partial least squares regression, the spectral data were then related to the Mankin scores of the samples tested.ResultsMild to severe degenerative cartilage changes were observed in the subject animals. The ACLT models showed mild cartilage degeneration, MSX models moderate, and MIA severe cartilage degenerative changes both morphologically and histologically. Our result demonstrates that NIR spectroscopic information is capable of separating the cartilage samples into different groups relative to the severity of degeneration, with NIR correlating significantly with their Mankin score (R2 = 88.85%).ConclusionWe conclude that NIR is a viable tool for evaluating articular cartilage health and physical properties such as change in thickness with degeneration.
Journal: Osteoarthritis and Cartilage - Volume 20, Issue 11, November 2012, Pages 1367–1373