کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3380887 1220225 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential response of porcine osteoblasts and chondrocytes in cell or tissue culture after 5-aminolevulinic acid-based photodynamic therapy
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی ایمونولوژی، آلرژی و روماتولوژی
پیش نمایش صفحه اول مقاله
Differential response of porcine osteoblasts and chondrocytes in cell or tissue culture after 5-aminolevulinic acid-based photodynamic therapy
چکیده انگلیسی

SummaryObjectiveOutcome in osteochondral allografting is limited by the immunological incompatibility of the grafted tissue. Based on a resistance of chondrocytes to photodynamic therapy in cell culture it is proposed that 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) might be used to inactivate bone while maintaining viability of chondrocytes and thus immunomodulate bone selectively.MethodsChondrocytes and osteoblasts from porcine humeral heads were either isolated (cell culture) or treated in situ (tissue culture). To quantify cytotoxic effects of 5-ALA-PDT (0–20 J/cm2, 100 mW/cm2) an (3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide) (MTT)-assay was used in cell culture and in situ hybridization in tissue culture to assess metabolic active cells (functional osteoblasts: colα1(I) mRNA, functional chondrocytes: colα1(II) mRNA).ResultsIn cell culture, survival after 5-ALA-PDT was significantly higher for chondrocytes (5 J/cm2: 87 ± 12% compared to untreated cells) than for osteoblasts (5 J/cm2: 12 ± 11%). In tissue culture, the percentage of functional chondrocytes in cartilage showed a decrease after 5-ALA-PDT (direct fixation: 92 ± 2%, 20 J/cm2: 35 ± 15%; P < 0.0001). A significant decrease in the percentage of bone surfaces covered by functional osteoblasts was observed in freshly harvested (31 ± 3%) compared to untreated tissues maintained in culture (11 ± 4%, P < 0.0001), with no further decrease after 5-ALA-PDT.ConclusionChondrocytes were more resistant to 5-ALA-PDT than osteoblasts in cell culture, while in tissue culture a loss of functional chondrocytes was observed after 5-ALA-PDT. Since osteoblasts - but not chondrocytes - were sensitive to the tissue culture conditions, devitalized bone with functional cartilage might already be achieved by applying specific tissue culture conditions even without 5-ALA-PDT.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Osteoarthritis and Cartilage - Volume 17, Issue 4, April 2009, Pages 539–546
نویسندگان
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